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Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor
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Image Search Results
Figure S4 . " width="100%" height="100%">
Journal: iScience
Article Title: Epithelial restitution in 3D - Revealing biomechanical and physiochemical dynamics in intestinal organoids via fs laser nanosurgery
doi: 10.1016/j.isci.2023.108139
Figure Lengend Snippet: Single-cell ablation in the crypt base leads to increased YAP signal in the surrounding cells To show the localization and expression of YAP1, a lesion was created and the colonoid was fixed and immunostained 30 min after ablation. (A) Phalloidin staining (phalloidin-Atto 488, Sigma Aldrich, St. Louis, MO, USA) to visualize the F-actin structures within the crypts. (B) Helix Blue Staining (Helix NP Blue, Biolegend, San Diego, CA, USA) was used for the visualization of the cell nuclei. (C) Merge of phalloidin-488, Helix Blue, and YAP1 staining. (D) YAP1 staining indicates the expression and localization of YAP1 within the damaged and restituting crypts. The region of interest is marked with a dashed line. 5 single cell ablation scenarios were evaluated and immunostained. (E) Interplay of the Wnt and Hippo-YAP/TAZ pathways. When the Wnt pathway is active, YAP/TAZ is released from the degradation complex. TAZ degradation depends on the phosphorylation of β-catenin. , β-catenin can escape the degradation complex when Wnt is active, translocates to the nucleus, and initiates the transcription of various genes via interaction with LEF/TEF. , Based on the escape of β-catenin, YAP/TAZ translocates into the nucleus and activates Wnt/β-catenin-dependent transcription. , Scale bar corresponds to 25 μm. See also
Article Snippet: Mouse monoclonal anti-YAP1 ,
Techniques: Expressing, Staining, Phospho-proteomics
Journal: iScience
Article Title: Epithelial restitution in 3D - Revealing biomechanical and physiochemical dynamics in intestinal organoids via fs laser nanosurgery
doi: 10.1016/j.isci.2023.108139
Figure Lengend Snippet:
Article Snippet: Mouse monoclonal anti-YAP1 ,
Techniques: Virus, Recombinant, Plasmid Preparation, Software, Membrane